Mycobacterial Lipids Induce Calcium Mobilization and Degranulation of Mast Cells
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Cells and culture conditions. Murine MCs from line C1.MC/C57.1 (C57 MC) were kindly provided by Dr. Stephen Galli (Stanford University) and cultured in Dulbecco’s modified Eagle’s medium according to standard methods (9). M. tuberculosis lipid extracts. The bacterial lipid fractions extracted from H37Rv M. tuberculosis, including total lipids (T-L, NR-14837), insoluble lipids (I-L, NR-14843), soluble lipids (S-L, NR-14842), M. leprae, phenolic glycolipid I (PGL-I, NR-19342), and M. tuberculosis phosphatidylinositol mannoside 6 (PIM6, NR-14847), were obtained from BEI Resources. Purified bovine heart L-a-phosphatidylcholine (PC, 840052C) and cardiolipin (CL, 840012C) were obtained from Avanti Polar Lipids. All M. tuberculosis lipid extracts were resuspended according to the manufacturer’s recommendations.