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dc.contributor.authorLai, Min
dc.contributor.authorHermann, Christopher D.
dc.contributor.authorCheng, Alice
dc.contributor.authorOlivares-Navarrete, Rene
dc.contributor.authorGittens, Rolando A.
dc.contributor.authorBird, Meredith M.
dc.contributor.authorWalker, Marcus
dc.contributor.authorCai, Ye
dc.contributor.authorCai, Kaiyong
dc.contributor.authorSandhage, Kenneth H.
dc.contributor.authorSchwartz, Zvi
dc.contributor.authorBoyan, Barbara D.
dc.date.accessioned2020-08-12T14:46:27Z
dc.date.available2020-08-12T14:46:27Z
dc.date.issued2014-05-07
dc.identifier.other10.1002/jbm.a.35185
dc.identifier.urihttp://repositorio-indicasat.org.pa/handle/123456789/236
dc.descriptionSurface microroughness plays an important role in determining osteoblast behavior on titanium. Previous studies have shown that osteoblast differentiation on microtextured titanium substrates is dependent on alpha-2 beta-1 (a2b1) integrin signaling. This study used focused ion beam milling and scanning electron microscopy, combined with three-dimensional image reconstruction, to investigate early interactions of individual cells with their substrate and the role of integrin a2b1 in determining cell shape. MG63 osteoblast-like cells on sand blasted/acid etched (SLA) Ti surfaces after 3 days of culturing indicated decreased cell number, increased cell differentiation, and increased expression of mRNA levels for a1, a2, aV, and b1 integrin subunits compared to cells on smooth Ti (PT) surfaces. a2 or b1 silenced cells exhibited increased cell number and decreased differentiation on SLA compared to wild-type cells. Wild-type cells on SLA possessed an elongated morphology with reduced cell area, increased cell thickness, and more apparent contact points. Cells on PT exhibited greater spreading and were relatively flat. Silenced cells possessed a morphology and phenotype similar to wild-type cells grown on PT. These observations indicate that surface microroughness affects cell response via a2b1 integrin signaling, resulting in a cell shape that promotes osteoblastic differentiation. VC 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 564–573, 2015.en_US
dc.description.abstractSurface microroughness plays an important role in determining osteoblast behavior on titanium. Previous studies have shown that osteoblast differentiation on microtextured titanium substrates is dependent on alpha-2 beta-1 (a2b1) integrin signaling. This study used focused ion beam milling and scanning electron microscopy, combined with three-dimensional image reconstruction, to investigate early interactions of individual cells with their substrate and the role of integrin a2b1 in determining cell shape. MG63 osteoblast-like cells on sand blasted/acid etched (SLA) Ti surfaces after 3 days of culturing indicated decreased cell number, increased cell differentiation, and increased expression of mRNA levels for a1, a2, aV, and b1 integrin subunits compared to cells on smooth Ti (PT) surfaces. a2 or b1 silenced cells exhibited increased cell number and decreased differentiation on SLA compared to wild-type cells. Wild-type cells on SLA possessed an elongated morphology with reduced cell area, increased cell thickness, and more apparent contact points. Cells on PT exhibited greater spreading and were relatively flat. Silenced cells possessed a morphology and phenotype similar to wild-type cells grown on PT. These observations indicate that surface microroughness affects cell response via a2b1 integrin signaling, resulting in a cell shape that promotes osteoblastic differentiation. VC 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 564–573, 2015.en_US
dc.formatapplication/pdf
dc.language.isoengen_US
dc.rightsInfo:eu-repo/semantics/openAccess
dc.rightshttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectosteoblasten_US
dc.subjectsurface microroughnessen_US
dc.subject3D reconstructionen_US
dc.subjectfocused ion beamen_US
dc.subjecta2b1 integrinen_US
dc.subjectcell morphologyen_US
dc.titleRole of a2b1 integrins in mediating cell shape on microtextured titanium surfacesen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeInfo:eu-repo/semantics/publishedversion


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