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dc.contributor.authorTayler, Nicole M.
dc.contributor.authorBoya, Cristopher A.
dc.contributor.authorHerrera, Liuris
dc.contributor.authorMoy, Jamie
dc.contributor.authorNg, Michelle
dc.contributor.authorPineda, Laura
dc.contributor.authorAlmanza, Alejandro
dc.contributor.authorRosero, Sara
dc.contributor.authorCoronado, Lorena M.
dc.contributor.authorCorrea, Ricardo
dc.contributor.authorSantamaría, Ricardo
dc.contributor.authorCaballero, Zuleima
dc.contributor.authorDurant-Archibold, Armando A.
dc.contributor.authorTidgewell, Kevin J.
dc.contributor.authorBalunas, Marcy J.
dc.contributor.authorGerwick, William H.
dc.contributor.authorSpadafora, Alida
dc.contributor.authorGutiérrez, Marcelino
dc.contributor.authorSpadafora, Carmenza
dc.date.accessioned2020-05-27T22:58:02Z
dc.date.available2020-05-27T22:58:02Z
dc.date.issued2019-04-02
dc.identifier.otherhttps://doi.org/10.1371/journal.pone.0214193
dc.identifier.urihttp://repositorio-indicasat.org.pa/handle/123456789/30
dc.descriptionCocos nucifera (C. nucifera) (the coconut palm tree) has been traditionally used to fight a number of human diseases, but only a few studies have tested its components against parasites such as those that cause malaria. In this study, C. nucifera samples were collected from a private natural reserve in Punta Patiño, Darien, Panama. The husk, leaves, pulp, and milk of C. nucifera were extracted and evaluated against the parasites that cause Chagas’ disease or American trypanosomiasis (Trypanosoma cruzi), leishmaniasis (Leishmania donovani) and malaria (Plasmodium falciparum), as well as against a line of breast cancer cells. While there was no activity in the rest of the tests, five and fifteen-minute aqueous decoctions of leaves showed antiplasmodial activity at 10% v/v concentration. Removal of some HPLC fractions resulted in loss of activity, pointing to the presence of synergy between the components of the decoction. Chemical molecules were separated and identified using an ultra-performance liquid chromatography (UPLC) approach coupled to tandem mass spectrometry (LC–MS/MS) using atmospheric pressure chemical ionization quadrupole–time of flight mass spectrometry (APCI–Q–TOF–MS) and molecular networking analysis, revealing the presence of compounds including polyphenol, flavone, sterol, fatty acid and chlorophyll families, among others.en_US
dc.description.abstractCocos nucifera (C. nucifera) (the coconut palm tree) has been traditionally used to fight a number of human diseases, but only a few studies have tested its components against parasites such as those that cause malaria. In this study, C. nucifera samples were collected from a private natural reserve in Punta Patiño, Darien, Panama. The husk, leaves, pulp, and milk of C. nucifera were extracted and evaluated against the parasites that cause Chagas’ disease or American trypanosomiasis (Trypanosoma cruzi), leishmaniasis (Leishmania donovani) and malaria (Plasmodium falciparum), as well as against a line of breast cancer cells. While there was no activity in the rest of the tests, five and fifteen-minute aqueous decoctions of leaves showed antiplasmodial activity at 10% v/v concentration. Removal of some HPLC fractions resulted in loss of activity, pointing to the presence of synergy between the components of the decoction. Chemical molecules were separated and identified using an ultra-performance liquid chromatography (UPLC) approach coupled to tandem mass spectrometry (LC–MS/MS) using atmospheric pressure chemical ionization quadrupole–time of flight mass spectrometry (APCI–Q–TOF–MS) and molecular networking analysis, revealing the presence of compounds including polyphenol, flavone, sterol, fatty acid and chlorophyll families, among others.en_US
dc.language.isoenen_US
dc.subjectcoconut palmen_US
dc.subjectPlasmodium falciparumen_US
dc.subjectTrypanosoma cruzien_US
dc.subjectLeishmania donovanien_US
dc.subjectChemical moleculesen_US
dc.subjectC. nuciferaen_US
dc.subjectmalariaen_US
dc.subjectNatural Reserveen_US
dc.titleAnalysis of the antiparasitic and anticancer activity of the coconut palm (Cocos nucifera L. ARECACEAE) from the natural reserve of Punta Patiño, Dariénen_US
dc.typeArticleen_US


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