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dc.contributor.authorAlmeida, C.
dc.contributor.authorOrtega, H.
dc.contributor.authorHigginbotham, S.
dc.contributor.authorSpadafora, C.
dc.contributor.authorArnold, A.E.
dc.contributor.authorColey, P.D.
dc.contributor.authorKursar, T.A.
dc.contributor.authorGerwick, W.H.
dc.contributor.authorCubilla-Rios, L.
dc.date.accessioned2020-06-23T03:57:40Z
dc.date.available2020-06-23T03:57:40Z
dc.date.issued2014-03-04
dc.identifier.issnISSN 0266-8254
dc.identifier.otherdoi:10.1111/lam.12245
dc.identifier.urihttp://repositorio-indicasat.org.pa/handle/123456789/104
dc.descriptionIn screening for natural products with antiparasitic activity, an endophytic fungus, strain F2611, isolated from above-ground tissue of the tropical grass Paspalum conjugatum (Poaceae) in Panama, was chosen for bioactive principle elucidation. Cultivation on malt extract agar (MEA) followed by bioassayguided chromatographic fractionation of the extract led to the isolation of the new polyketide integrasone B (1) and two known mycotoxins, sterigmatocystin (2) and secosterigmatocystin (3). Sterigmatocystin (2) was found to be the main antiparasitic compound in the fermentation extract of this fungus, possessing potent and selective antiparasitic activity against Trypanosoma cruzi, the cause of Chagas disease, with an IC50 value of 0 13 lmol l1 . Compounds 2 and 3 showed high cytotoxicity against Vero cells (IC50 of 0 06 and 0 97 lmol l1 , respectively). The new natural product integrasone B (1), which was co-purified from the active fractions, constitutes the second report of a natural product possessing an epoxyquinone with a lactone ring and exhibited no significant biological activity. Strain F2611 represents a previously undescribed taxon within the Microthyriaceae (Dothideomycetes, Ascomycota)en_US
dc.description.abstractIn screening for natural products with antiparasitic activity, an endophytic fungus, strain F2611, isolated from above-ground tissue of the tropical grass Paspalum conjugatum (Poaceae) in Panama, was chosen for bioactive principle elucidation. Cultivation on malt extract agar (MEA) followed by bioassayguided chromatographic fractionation of the extract led to the isolation of the new polyketide integrasone B (1) and two known mycotoxins, sterigmatocystin (2) and secosterigmatocystin (3). Sterigmatocystin (2) was found to be the main antiparasitic compound in the fermentation extract of this fungus, possessing potent and selective antiparasitic activity against Trypanosoma cruzi, the cause of Chagas disease, with an IC50 value of 0 13 lmol l1 . Compounds 2 and 3 showed high cytotoxicity against Vero cells (IC50 of 0 06 and 0 97 lmol l1 , respectively). The new natural product integrasone B (1), which was co-purified from the active fractions, constitutes the second report of a natural product possessing an epoxyquinone with a lactone ring and exhibited no significant biological activity. Strain F2611 represents a previously undescribed taxon within the Microthyriaceae (Dothideomycetes, Ascomycota)en_US
dc.formatapplication/pdf
dc.language.isoengen_US
dc.rightsInfo:eu-repo/semantics/openAccess
dc.rightshttps://creativecommons.org/licenses/by/4.0/
dc.subjectantiparasitic activityen_US
dc.subjectendophytic fungien_US
dc.subjectintegrasone Ben_US
dc.subjectsterigmatocystin.en_US
dc.titleChemical and bioactive natural products from Microthyriaceae sp., an endophytic fungus from a tropical grassen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dcterms.typeInfo:eu-repo/semantics/publishedversion


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