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dc.contributor.authorChristian, Natalie
dc.contributor.authorSedio, Brian E.
dc.contributor.authorFlorez-Buitrago, Ximena
dc.contributor.authorRamírez-Camejo, Luis A.
dc.contributor.authorRojas, Enith I.
dc.contributor.authorMejía, Luis C.
dc.contributor.authorPalmedo, Sage
dc.contributor.authorRose, Autumn
dc.contributor.authorSchroeder, John W.
dc.contributor.authorHerre, Edward Allen
dc.date.accessioned2020-07-07T04:29:50Z
dc.date.available2020-07-07T04:29:50Z
dc.date.issued2019-11-23
dc.identifier.otherdoi:10.1002/ajb2.1436
dc.identifier.urihttp://repositorio-indicasat.org.pa/handle/123456789/176
dc.descriptionPREMISE: Interactions between fungal endophytes and their host plants present useful systems for identifying important factors affecting assembly of host-associated microbiomes. Here we investigated the role of secondary chemistry in mediating host affinity of asymptomatic foliar endophytic fungi using Psychotria spp. and Theobroma cacao (cacao) as hosts. METHODS: First, we surveyed endophytic communities in Psychotria species in a natural common garden using culture-based methods. Then we compared differences in endophytic community composition with differences in foliar secondary chemistry in the same host species, determined by liquid chromatography–tandem mass spectrometry. Finally, we tested how inoculation with live and heat-killed endophytes affected the cacao chemical profile. RESULTS: Despite sharing a common environment and source pool for endophyte spores, different Psychotria host species harbored strikingly different endophytic communities that reflected intrinsic differences in their leaf chemical profiles. In T. cacao, inoculation with live and heat-killed endophytes produced distinct cacao chemical profiles not found in uninoculated plants or pure fungal cultures, suggesting that endophytes, like pathogens, induce changes in secondary chemical profiles of their host plant. CONCLUSIONS: Collectively our results suggest at least two potential processes: (1) Plant secondary chemistry influences assembly and composition of fungal endophytic communities, and (2) host colonization by endophytes subsequently induces changes in the host chemical landscape. We propose a series of testable predictions based on the possibility that reciprocal chemical interactions are a general property of plant–endophyte interactionsen_US
dc.description.abstractPREMISE: Interactions between fungal endophytes and their host plants present useful systems for identifying important factors affecting assembly of host-associated microbiomes. Here we investigated the role of secondary chemistry in mediating host affinity of asymptomatic foliar endophytic fungi using Psychotria spp. and Theobroma cacao (cacao) as hosts. METHODS: First, we surveyed endophytic communities in Psychotria species in a natural common garden using culture-based methods. Then we compared differences in endophytic community composition with differences in foliar secondary chemistry in the same host species, determined by liquid chromatography–tandem mass spectrometry. Finally, we tested how inoculation with live and heat-killed endophytes affected the cacao chemical profile. RESULTS: Despite sharing a common environment and source pool for endophyte spores, different Psychotria host species harbored strikingly different endophytic communities that reflected intrinsic differences in their leaf chemical profiles. In T. cacao, inoculation with live and heat-killed endophytes produced distinct cacao chemical profiles not found in uninoculated plants or pure fungal cultures, suggesting that endophytes, like pathogens, induce changes in secondary chemical profiles of their host plant. CONCLUSIONS: Collectively our results suggest at least two potential processes: (1) Plant secondary chemistry influences assembly and composition of fungal endophytic communities, and (2) host colonization by endophytes subsequently induces changes in the host chemical landscape. We propose a series of testable predictions based on the possibility that reciprocal chemical interactions are a general property of plant–endophyte interactionsen_US
dc.formatapplication/pdf
dc.language.isoengen_US
dc.rightsInfo:eu-repo/semantics/openAccess
dc.rightshttps://creativecommons.org/licenses/by/4.0/
dc.subjectCommunity assemblyen_US
dc.subjectmetabolomicsen_US
dc.subjectmicrobiomeen_US
dc.subjectPsychotriaen_US
dc.subjectspecies specificityen_US
dc.subjectTheobroma cacaoen_US
dc.titleHost affinity of endophytic fungi and the potential for reciprocal interactions involving host secondary chemistryen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dcterms.typeInfo:eu-repo/semantics/publishedversion


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