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dc.contributor.authorDomínguez, Juan
dc.contributor.authorAcosta, Fermín
dc.contributor.authorPérez-Lago, Laura
dc.contributor.authorSambrano, Dilcia
dc.contributor.authorBatista, Victoria
dc.contributor.authorDe La Guardia, Carolina
dc.contributor.authorAbascal, Estefanía
dc.contributor.authorChiner-Oms, Álvaro
dc.contributor.authorComas, Iñaki
dc.contributor.authorGonzález, Prudencio
dc.contributor.authorBravo, Jaime
dc.contributor.authorDel Cid, Pedro
dc.contributor.authorRosas, Samantha
dc.contributor.authorMuñoz, Patricia
dc.contributor.authorGoodridge, Amador
dc.contributor.authorGarcía de Viedma, Darío
dc.date.accessioned2020-08-12T22:36:56Z
dc.date.available2020-08-12T22:36:56Z
dc.date.issued2019-03-01
dc.identifier.other10.3201/eid2503.181593
dc.identifier.urihttp://repositorio-indicasat.org.pa/handle/123456789/237
dc.descriptionSystematic molecular/genomic epidemiology studies for tuberculosis surveillance cannot be implemented in many countries. We selected Panama as a model for an alternative strategy. Mycobacterial interspersed repetitive unit–variable-number tandem-repeat (MIRU-VNTR) analysis revealed a high proportion (50%) of Mycobacterium tuberculosis isolates included in 6 clusters (A–F) in 2 provinces (Panama and Colon). Cluster A corresponded to the Beijing sublineage. Whole-genome sequencing (WGS) differentiated clusters due to active recent transmission, with low single-nucleotide polymorphism–based diversity (cluster C), from clusters involving long-term prevalent strains with higher diversity (clusters A, B). Prospective application in Panama of 3 tailored strain–specific PCRs targeting marker single-nucleotide polymorphisms identified from WGS data revealed that 31.4% of incident cases involved strains A–C and that the Beijing strain was highly represented and restricted mainly to Colon. Rational integration of MIRU-VNTR, WGS, and tailored strain–specific PCRs could be a new model for tuberculosis surveillance in countries without molecular/genomic epidemiology programs.en_US
dc.description.abstractSystematic molecular/genomic epidemiology studies for tuberculosis surveillance cannot be implemented in many countries. We selected Panama as a model for an alternative strategy. Mycobacterial interspersed repetitive unit–variable-number tandem-repeat (MIRU-VNTR) analysis revealed a high proportion (50%) of Mycobacterium tuberculosis isolates included in 6 clusters (A–F) in 2 provinces (Panama and Colon). Cluster A corresponded to the Beijing sublineage. Whole-genome sequencing (WGS) differentiated clusters due to active recent transmission, with low single-nucleotide polymorphism–based diversity (cluster C), from clusters involving long-term prevalent strains with higher diversity (clusters A, B). Prospective application in Panama of 3 tailored strain–specific PCRs targeting marker single-nucleotide polymorphisms identified from WGS data revealed that 31.4% of incident cases involved strains A–C and that the Beijing strain was highly represented and restricted mainly to Colon. Rational integration of MIRU-VNTR, WGS, and tailored strain–specific PCRs could be a new model for tuberculosis surveillance in countries without molecular/genomic epidemiology programs.en_US
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTuberculosisen_US
dc.subjectwhole-genome sequencingen_US
dc.subjectstrain-specific PCRen_US
dc.subjectPanamaen_US
dc.subjecttransmissionen_US
dc.subjectepidemiologyen_US
dc.subjecttuberculosis and other mycobacteriaen_US
dc.subjectbacteriaen_US
dc.subjectTBen_US
dc.subjectmodelen_US
dc.subjectsurveillanceen_US
dc.titleSimplified Model to Survey Tuberculosis Transmission in Countries Without Systematic Molecular Epidemiology Programsen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeinfo:edu-repo/semantics/publishedVersion


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